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TLS Online TPP Program
#Question id: 13058
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?
TLS Online TPP Program
#Question id: 2969
#SCPH01 Biochemistry
In the cells of many eukaryotic species, the nuclear envelope has to disappear to permit which of the following events in the cell cycle?
TLS Online TPP Program
#Question id: 3708
#SCPH28 | Zoology
The incorporation of new nucleotides into a growing strand of DNA occurs during ________.
TLS Online TPP Program
#Question id: 640
#SCPH05 I Biotechnology
The data in the table above were collected for an enzyme-catalyzed reaction. The Km for this enzyme is approximately
TLS Online TPP Program
#Question id: 17655
#SCPH05 I Biotechnology
In DNA isolation, for protein free solution what will be the ratio at nm