In a maternity ward, four babies become accidentally mixed up. The ABO types of the three babies are known to be A, B, and AB. The ABO types of the four sets of parents that are clamed are determined.

(A) AB X O  (B) A X O, (C) A X AB, (D) B  X O.

Following some statement is given

A. A blood group baby must be child of Parent B

B. B blood group baby must be child of Parent A

C. AB blood group baby must be child of Parent A

D. AB blood group baby must be child of Parent C

Which of the following above statement is correct?

#Question id: 14272

#SCPH05 I Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P₀ = 0.1 g/l, determine the product concentration in the vessel at t = 10 h

#Question id: 14273

#SCPH05 I Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d^-1 and the yield of biomass from substrate is 0.5 g g^-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l^{- 1} cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d^-1. Fed-batch operation occurs under quasi-steady-state conditions. Estimate the batch culture time.                                  ____________________

#Question id: 14274

#SCPH05 I Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d^-1 and the yield of biomass from substrate is 0.5 g g^-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l^{- 1} cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d^-1. Fed-batch operation occurs under quasi-steady-state conditions. Estimate final biomass concentration?

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#Question id: 14277

#SCPH05 I Biotechnology

Lactobacillus casei is propagated under essentially anaerobic conditions to provide a starter culture for manufacture of Swiss cheese. The culture produces lactic acid as a by-product of energy metabolism. The system has the following characteristics:

A stirred fermenter is operated in fed-batch mode at quasisteady state with a feed flow rate of 4 m³ h^-1 and feed substrate concentration of 80 kg m^-3. After 6 h, the liquid volume is 40 m³. What mass of cells is produced after 6 h fed-batch operation?

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