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TLS Online TPP Program
#Question id: 13061
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Arrange in the order of their increasing capacity:
TLS Online TPP Program
#Question id: 15208
#SCPH01 Biochemistry
The best result of band pattern can be observed by adjusting
TLS Online TPP Program
#Question id: 8887
#SCPH06 I Botany
Generally, members of which flatworm class(es) are nonparasitic?
TLS Online TPP Program
#Question id: 3879
#SCPH05 I Biotechnology
Which of the following is not usually essential for the catalytic activity of ribozymes?
TLS Online TPP Program
#Question id: 4720
#SCPH01 Biochemistry
Which of the following phenomenon, the differential expression of genetic material depending on whether it is inherited from the male or female parent, is called