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TLS Online TPP Program
#Question id: 13054
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 371
#SCPH28 | Zoology
How many milliliters of a 2.0 M solution of boric acid must be added to 600 mL of a solution of 10 mM sodium borate in order for the pH to be 9.45? (pKa of boric acid = 9.24)
TLS Online TPP Program
#Question id: 5688
#SCPH28 | Zoology
Which of the following are not cause of aneuploidy
TLS Online TPP Program
#Question id: 856
#SCPH06 I Botany
Protein folding is not a completely random, trial-and-error process, This problem was first pointed out by
TLS Online TPP Program
#Question id: 19512
#SCPH05 I Biotechnology
In a group of the periodic table the Ionization enthalpies of the elements decreases from top to bottom because of ———-