What do animals as diverse as corals and monkeys have in common?

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#Question id: 18895

#SCPH01 Biochemistry

Degree of scattering in transmission electron microscope is a function of

TLS Online TPP Program

#Question id: 11097

#I Life Science/ Life Sciences Group – I-V

The volume of air in the lungs following a complete exhalation of the expiratory reserve volume is

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#Question id: 7752

#I Life Science/ Life Sciences Group – I-V

The autophagy pathway included____

a) the catabolic mechanism that delivers cellular components to lysosomes, where they are degraded

b) Autophagy protects the cell from the harmful or lethal effects of damaged or unnecessary proteins and organelles

c) During starvation, the autophagic breakdown and recycling of cellular components also

ensure cellular survival by maintaining cellular energy levels

Which of the following are the correct prediction about autophagy is true?

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#Question id: 12733

#SCPH06 I Botany

It provides a key marker of potential disease development and reaction to individual treatment.

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#Question id: 13091

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?