#Question id: 14282
#SCPH05 I Biotechnology
#Question id: 15649
#SCPH05 I Biotechnology
#Question id: 45
#I Life Science/ Life Sciences Group – I-V
What is the standard free-energy change, ΔG°, under physiological conditions (E. coli grows in the human gut, at 37 °C) for the following reaction? (Keq = 7.8 × 10^2)
Glucose + ATP → glucose 6-phosphate + ADP
#Question id: 9162
#SCPH06 I Botany
If the direction of Earth's rotation reversed, the most predictable effect would be ________.
#Question id: 13076
#SCPH05 I Biotechnology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.