#Question id: 568
#SCPH05 I Biotechnology
Which of the following statements about allosteric control of enzymatic activity is false?
#Question id: 11953
#I Life Science/ Life Sciences Group – I-V
Which of the following is a difference between vitamins and minerals?
#Question id: 660
#SCPH05 I Biotechnology
Computers are used to advance the understanding of three dimensional protein structure by
#Question id: 1404
#SCPH28 | Zoology
Which of the following are transcription factors?
#Question id: 643
#SCPH05 I Biotechnology
The hydrolysis of lactose is catalysed by b-galactosidase as given below
Which of the following could be correct mechanism for this hydrolysis reaction?
A. Nucleophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
B. Electrophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
C. Nucleophilic attack by the carboxylate group of Lys/Arg/His or bivalent cation on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.