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TLS Online TPP Program
#Question id: 1718
#SCPH05 I Biotechnology
When Langerhans' cells differentiate into fully mature dendritic cells (DCs) they:
TLS Online TPP Program
#Question id: 15617
#SCPH06 I Botany
Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;
TLS Online TPP Program
#Question id: 13064
#SCPH05 I Biotechnology
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following techniques doesn’t involve use of a secondary antibody?
TLS Online TPP Program
#Question id: 14662
#SCPH05 I Biotechnology
The laboratory work using computers and associated with web-based analysis generally online is referred to as __________.
TLS Online TPP Program
#Question id: 1599
#SCPH05 I Biotechnology
Name the class of MHC which is recognized by CD4 TH cell.