Which one of these processes describes bottleneck effect?

TLS Online TPP Program

#Question id: 13092

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 15616

#SCPH28 | Zoology

The diagram below shows the F factor and a portion of the E. coli chromosome that has three different insertion sequences (IS) of the same type as is carried on F.

                     

Describe the three different Hfrs (Hfr #1, Hfr #2 and Hfr #3) that can be formed by recombination between the IS on F and each of the IS sequences on the chromosome. Some including the positions of each of the markers (A, B, C, and D) and state which of these markers would be transferred early?

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#Question id: 11133

#SCPH28 | Zoology

Which of the following statements accurately describes a feature of temperature sensation by the nervous system?

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#Question id: 18011

#SCPH01 Biochemistry

ExPASY  is 

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#Question id: 15620

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition Lac– strain, called lac3–, is linked to the Tn5 insertion. From a strain carrying the Tn5 insertion and lac3– mutation you isolate an F’ that caries a region of the chromosome that includes both Tn5 and the linked Lac region. Introduce this F’ into an F– strain carrying lac1– by selecting for Kanr. These merodiploids express ß-galactosidase normally. What does this result tell you about the relationship between the lac3– and lac1- mutations?