Animal preference of polygyny mating systems because of

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#Question id: 12932

#SCPH05 I Biotechnology

Why mRNA cannot be used directly in cloning?

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#Question id: 1569

#SCPH05 I Biotechnology

A bacterium entering the body through a small cut in the skin will do which of the following?

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#Question id: 9177

#SCPH28 | Zoology

Which of the following statements regarding turnover in a lake is correct?

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#Question id: 936

#I Life Science/ Life Sciences Group – I-V

At neutral pH, the main ionic form of ammonia is ________.

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#Question id: 13088

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;