TLS Online TPP Program
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TLS Online TPP Program
#Question id: 4944
#SCPH06 I Botany
What is true of the amino acids that might have been delivered to Earth within carbonaceous chondrites?
TLS Online TPP Program
#Question id: 14345
#SCPH05 I Biotechnology
washout phenomenon occurs in reactor when
TLS Online TPP Program
#Question id: 17206
#SCPH05 I Biotechnology
In regular expressions, which of the following pair of pattern is wrongly matched with its significance?
TLS Online TPP Program
#Question id: 16101
#SCPH28 | Zoology
The set-point temperature of the hypothalamus triggering heat loss or heat generating mechanisms is
TLS Online TPP Program
#Question id: 13087
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?