According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

#Question id: 4940

#SCPH28 | Zoology

In their laboratory simulations of the early Earth, Miller and Urey observed the abiotic synthesis of

#Question id: 4941

#SCPH06 I Botany

Which of the factors below weaken the hypothesis of abiotic synthesis of organic monomers in early Earthʹs atmosphere?

1.  the relatively short time between intense meteor bombardment and appearance of the first life forms

2.  the lack of experimental evidence that organic monomers can form by abiotic synthesis

3.  Uncertainty about which gases comprised early Earthʹs atmosphere

#Question id: 4941

#SCPH28 | Zoology

Which of the factors below weaken the hypothesis of abiotic synthesis of organic monomers in early Earthʹs atmosphere?

1.  the relatively short time between intense meteor bombardment and appearance of the first life forms

2.  the lack of experimental evidence that organic monomers can form by abiotic synthesis

3.  Uncertainty about which gases comprised early Earthʹs atmosphere

#Question id: 4942

#SCPH06 I Botany

Which of the following has not yet been synthesized in laboratory experiments studying the origin of life?

#Question id: 4942

#SCPH28 | Zoology

Which of the following has not yet been synthesized in laboratory experiments studying the origin of life?

#Question id: 4943

#SCPH06 I Botany

In what way were conditions on the early Earth of more than 3 billion years ago different from those on todayʹs Earth?