According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

TLS Online TPP Program

#Question id: 4332

#SCPH01 Biochemistry

Which of the following is not true of a codon?

TLS Online TPP Program

#Question id: 15602

#SCPH28 | Zoology

The antibiotic erythromycin, the immunosuppressive drug FK506, and the cholesterol-lowering drug lovastatin all belong to which class.

TLS Online TPP Program

#Question id: 2603

#I Life Science/ Life Sciences Group – I-V

If no ribosome begins translation of the leader peptide AUG, the hairpin forms by pairing of sequences 1 and 2, preventing formation of the 2 –3 hairpin, and allowing formation of the hairpin at sequences 3 –4, the condition is

TLS Online TPP Program

#Question id: 5070

#SCPH01 Biochemistry

During juxtacrine pathway, notch protein is activated by the neighbouring cells which expresses:

TLS Online TPP Program

#Question id: 11802

#SCPH05 I Biotechnology

Damaged sieve elements are sealed off, but because of the processs that plug sieve plates pores, excuded phloem sap from wounds that sever sieve elements. Exudation of sap enhanced by the inclusion of;