Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 22940

#SCPH28 | Zoology

In rice, a SDP, homolog of CONSTANS gene is 

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#Question id: 10878

#SCPH28 | Zoology

The resistance of the pulmonary tree is so low that a 1 cm of water pressure gradient is sufficient to cause normal air flow during resting conditions. Which of the following often has a substantial resistance during pulmonary disease states that can limit alveolar ventilation?

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#Question id: 365

#SCPH05 I Biotechnology

Water is a polar molecule: the oxygen atom with its unshared electrons carries a partial negative charge (δ−) of ___________, and the hydrogen atoms each carry a partial positive charge (δ+) of __________, where e is the charge of the electron.

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#Question id: 15142

#SCPH05 I Biotechnology

Dot matrix analysis of the amino acid sequences of lambda phage cI (horizontal sequence) and phage P22 c2 (vertical sequence) repressors is shown below. Which one of the following is correct?

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#Question id: 18639

#I Life Science/ Life Sciences Group – I-V

Which one of the following techniques is used for the evaluation of phylogenetic trees?