Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 2151

#SCPH28 | Zoology

Which of the following statement(s) is (are) true of transmembrane proteins?

a. All contain a hydrophobic α helix.

b. Some serve as membrane attachment sites for peripheral membrane proteins.

c. Some contain more than one membrane-spanning domain.

d. All are asymmetrically oriented in the lipid bilayer.

#Question id: 2152

#SCPH28 | Zoology

Precursors of membrane phospholipids are

a. soluble in the cytosol.                    

b. incorporated into biosynthetic intermediates.

c. sometimes complexed with fatty-acid-binding proteins.

d. stored in glycerolosomes.

e. sometimes activated by conjugation with CDP.

#Question id: 2153

#SCPH28 | Zoology

Membrane phospholipids include

a. cholesterol.            

b. plasmologens.        

c. sphingolipids.         

d. triglycerides.                      

e. bile acids.

#Question id: 2154

#SCPH28 | Zoology

Membrane lipids are distributed appropriately between the two leaflets of a given membrane by

a. dockases.    

b. trumpases.              

c. fatty acid desaturases.        

d. flippases.    

e. transmembrane tunnelases.

#Question id: 2155

#SCPH28 | Zoology

Cholesterol is

a. unrelated to any plant lipids.                     

b. not found in chloroplast membranes.

c. encoded by an intron-containing gene on chromosome 23.

d. relatively abundant in the plasma membrane and endocytic membranes.

e. synthesized in the ER.

#Question id: 2156

#SCPH28 | Zoology

Proteins known to be involved in Golgi apparatus independent transport of lipids include

a. StAR protein.                                 

b. Neimann-Pick C1 (NPC1) protein.

c. HMG CoA reductase.                    

d. acyl:cholesterol acyl transferase (ACAT).

e. cholesterol 7α-hydroxylase.