Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 15884

#SCPH05 I Biotechnology

Laminar airflow is used for the following reason except;

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#Question id: 24441

#SCPH01 Biochemistry

If the heart rate is 90 beats/min, what is the cycle length?

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#Question id: 16133

#SCPH06 I Botany

You are running a human assisted reproduction clinic and providing state-of-the-art genetic diagnostic services. A married couple who already had a child with cystic fibrosis approach you because they wish to have another child, but only if they can be assured that the child will not have cystic fibrosis. You genotype the woman and discover that she is a heterozygote for Del508, the most common mutation causing cystic fibrosis. You suggest that the couple consider first polar body testing, in which several unfertilized oocytes (each with its first polar body) are retrieved from the woman, the first polar bodies are removed, and PCR tests are conducted on DNA from each of the first polar bodies. The couple agrees, and you obtain the following results:

 

Given the couple's desire to have a child without cystic fibrosis, which oocytes would you employ in vitro fertilization?

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#Question id: 307

#SCPH01 Biochemistry

An ionic compound A+ B– is most likely to be formed when________

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#Question id: 4831

#SCPH12 I Genetics

There are 4 pairs of chromosomes in a Drosophila.The linkage groups present in it are