Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 4767

#SCPH12 I Genetics

Neurofibromatosis is a condition in humans which is generally inherited in a dominant fashion. Assuming this disorder is 90% penetrant, what is the chance that a single child born to a father with neurofibromatosis and a mother known not to carry the mutation will have the disorder?

TLS Online TPP Program

#Question id: 469

#SCPH05 I Biotechnology

The anaerobic conversion of 1 mol of glucose to 2 mol of lactate by fermentation is accompanied by a net gain of:

TLS Online TPP Program

#Question id: 1557

#SCPH05 I Biotechnology

which one of these antibody can cross placenta?

TLS Online TPP Program

#Question id: 350

#SCPH28 | Zoology

Cohesion, surface tension, and adhesion are the properties of water molecules that ________.

TLS Online TPP Program

#Question id: 14503

#SCPH28 | Zoology

If 10 coins are tossed 100 times, how many times would you expect 7 coins to fall head upward ?