Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

#Question id: 4756

#SCPH12 I Genetics

In summer squash, white colour fruit (W) is dominant over yellow colour (w) and disc-shaped phenotype (D) is dominant over sphere-shaped phenotype (d). Determine the genotype of the parents gives 3/8 white, sphere, 3/8 white, Disk, 1/8 Yellow, Disk and 1/8 Yellow, Sphere?

#Question id: 4757

#SCPH01 Biochemistry

The newborn baby of a mother having blood group O, Rh+ and father having blood group AB, Rh-, got mixed with other babies in the hospital. The baby with which of the following blood groups is not confirmable to be of the said couple?

(i) AB, Rh+                                                    (ii) O, Rh+

(iii) A, Rh+                                         (iv)  B, Rh+

#Question id: 4757

#SCPH12 I Genetics

The newborn baby of a mother having blood group O, Rh+ and father having blood group AB, Rh-, got mixed with other babies in the hospital. The baby with which of the following blood groups is not confirmable to be of the said couple?

(i) AB, Rh+                                                    (ii) O, Rh+

(iii) A, Rh+                                         (iv)  B, Rh+

#Question id: 4758

#SCPH01 Biochemistry

The allele l in human population is recessive lethal and x chromosome linked. If a female is heterozygous for l allele marries with normal male. What will be interpretation correct?

#Question id: 4758

#SCPH12 I Genetics

The allele l in human population is recessive lethal and x chromosome linked. If a female is heterozygous for l allele marries with normal male. What will be interpretation correct?

#Question id: 4759

#SCPH01 Biochemistry

which of the following is not a mechanism for sex determination?