Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 8886

#SCPH06 I Botany

Which characteristic is shared by both cnidarians and flatworms?

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#Question id: 3281

#SCPH06 I Botany

One population X has 750 AA, 0 Aa, and 250 aa; the other Y has 500 AA, 500 Aa, and 0 aa. After one generation of random mating, the genotype frequencies X and Y respectively if the population size remains 1,000.

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#Question id: 11422

#SCPH28 | Zoology

The values of temperature and precipitation used to define climate zones correspond to the conditions of moisture and cold stress that are particularly important determinants of plant form. Which of following is correct combination of Biome?

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#Question id: 3648

#SCPH06 I Botany

The initiation of DNA replication within a replicon always occurs at a fixed point known as:

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#Question id: 11001

#I Life Science/ Life Sciences Group – I-V

In which phase of the ventricular muscle action potential is the potassium permeability the highest?