Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

#Question id: 4745

#SCPH01 Biochemistry

In some goats, the presence of horns is produced by an autosomal gene that is dominant in males and recessive in females. A horned female is crossed with a hornless male. The F1 offspring are intercrossed to produce the F2. What proportion of the F2 offspring will have horns?

#Question id: 4745

#SCPH12 I Genetics

In some goats, the presence of horns is produced by an autosomal gene that is dominant in males and recessive in females. A horned female is crossed with a hornless male. The F1 offspring are intercrossed to produce the F2. What proportion of the F2 offspring will have horns?

#Question id: 4745

#SCPH28 | Zoology

In some goats, the presence of horns is produced by an autosomal gene that is dominant in males and recessive in females. A horned female is crossed with a hornless male. The F1 offspring are intercrossed to produce the F2. What proportion of the F2 offspring will have horns?

#Question id: 4746

#SCPH01 Biochemistry

Two independent pure line varieties having deep red and brown phenotype in their seed coat were cross, the all f1 progeny is deep red. The F2 progeny obtain from selfing show deep red and brown in ratio of 15:1. Which of the following observation is correct?

#Question id: 4746

#SCPH01 Biochemistry

Two independent pure line varieties having deep red and brown phenotype in their seed coat were cross, the all f1 progeny is deep red. The F2 progeny obtain from selfing show deep red and brown in ratio of 15:1. Which of the following observation is correct?

#Question id: 4746

#SCPH12 I Genetics

Two independent pure line varieties having deep red and brown phenotype in their seed coat were cross, the all f1 progeny is deep red. The F2 progeny obtain from selfing show deep red and brown in ratio of 15:1. Which of the following observation is correct?