Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

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#Question id: 5259

#SCPH05 I Biotechnology

Genetic variation in bacterial populations cannot result from

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#Question id: 4332

#SCPH06 I Botany

Which of the following is not true of a codon?

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#Question id: 3446

#SCPH06 I Botany

Which of the following is least related to the others?

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#Question id: 10280

#I Life Science/ Life Sciences Group – I-V

The enzyme of glycolytic pathway that forms fructose 1,6-bisphosphate is called phosphofructokinase-1 (PFK-1), which statements can’t distinguish it from a second enzyme (PFK-2) Phosphofructokinase-2.

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#Question id: 19629

#SCPH05 I Biotechnology

Le Chatelier’s principle is applicable to: