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TLS Online TPP Program

#Question id: 13066

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?

#SCPH28 | Zoology
  1. RFLP and AFLP
  2. RFLP and RAPD
  3. AFLP only
  4. RAPD only
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TLS Online TPP Program

#Question id: 12725

#SCPH05 I Biotechnology

Match the following

DNA form

Helix Direction

Number of bases per helix turn

1. B

a. Left Handed

i. 10

2.  A

b. Right Handed

ii. 12

3.  C

c. Left Handed

iii. 14

4. Z

d. Right Handed

iv. 9
TLS Online TPP Program

#Question id: 559

#SCPH05 I Biotechnology

In competitive inhibition, an inhibitor:
TLS Online TPP Program

#Question id: 5347

#SCPH06 I Botany

The inheritance of a given disorder is recorded in small families shown below: Based on the above limited information, which one of the following inheritance pattern best explains the observations?
TLS Online TPP Program

#Question id: 2614

#I Life Science/ Life Sciences Group – I-V

Effects in synthesis, if replaces trp codons with different amino acids;

His concentration low

Run/ On

Ala concentration High

Off

TLS Online TPP Program

#Question id: 14275

#SCPH05 I Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d-1 and the yield of biomass from substrate is 0.5 g g-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l- 1 cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d-1. Fed-batch operation occurs under quasi-steady-state conditions. Fed-batch operation is carried out for 40 d. What is the final mass of cells in the reactor?

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