Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 10885

#SCPH06 I Botany

In a field experiment, autotrophs are provided a 14C-labelled carbon compound for photosynthesis. Radioactivity (14C) levels were then monitored at regular intervals in all the trophic levels. In which ecosystem is the radioactivity likely to be detected in longer time at the higher trophic level?

#Question id: 10885

#SCPH28 | Zoology

In a field experiment, autotrophs are provided a 14C-labelled carbon compound for photosynthesis. Radioactivity (14C) levels were then monitored at regular intervals in all the trophic levels. In which ecosystem is the radioactivity likely to be detected in longer time at the higher trophic level?

#Question id: 10886

#SCPH06 I Botany

Much greater proportion of energy fixed by autotrophs is transferred to the herbivore level in the open ocean ecosystem than in a forest ecosystem because

#Question id: 10886

#SCPH28 | Zoology

Much greater proportion of energy fixed by autotrophs is transferred to the herbivore level in the open ocean ecosystem than in a forest ecosystem because

#Question id: 10887

#SCPH06 I Botany

What is a linkage density of community A and B RESPECTIVELY?

#Question id: 10887

#SCPH28 | Zoology

What is a linkage density of community A and B RESPECTIVELY?