To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 27163

#SCPH01 Biochemistry

which of the following database contains the information of regular expressions

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#Question id: 15376

#SCPH28 | Zoology

If a protein made up of three subunit α₂βγ (Mr. of α = 25000, β = 50000, γ = 75000) which of the following correctly representing SDS and Native (buffer) gel

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#Question id: 3664

#SCPH28 | Zoology

 If a mutation is complemented by a clone of a different gene whose product is being overproduced, this is called:

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#Question id: 17206

#SCPH01 Biochemistry

In regular expressions, which of the following pair of pattern is wrongly matched with its significance?

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#Question id: 14127

#SCPH05 I Biotechnology

The most basic sequence alignment method is –