To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 50

#SCPH05 I Biotechnology

The enzyme fumarase catalyzes the reverse hydration of fumaric acid to l-malate, but it will not catalyze the hydration of maleic acid, the cis isomer of fumaric acid. This is an example of:

#Question id: 50

#SCPH28 | Zoology

The enzyme fumarase catalyzes the reverse hydration of fumaric acid to l-malate, but it will not catalyze the hydration of maleic acid, the cis isomer of fumaric acid. This is an example of:

#Question id: 51

#SCPH01 Biochemistry

 If the equilibrium constant, Keq, for the reaction ATP → ADP + Pi is 2.22 × 10⁵ M, calculate the standard free-energy change, ΔG°, for the synthesis of ATP from ADP and Pi at 25 °C.

#Question id: 51

#I Life Science/ Life Sciences Group – I-V

 If the equilibrium constant, Keq, for the reaction ATP → ADP + Pi is 2.22 × 10⁵ M, calculate the standard free-energy change, ΔG°, for the synthesis of ATP from ADP and Pi at 25 °C.

#Question id: 51

#SCPH05 I Biotechnology

 If the equilibrium constant, Keq, for the reaction ATP → ADP + Pi is 2.22 × 10⁵ M, calculate the standard free-energy change, ΔG°, for the synthesis of ATP from ADP and Pi at 25 °C.

#Question id: 51

#SCPH28 | Zoology

 If the equilibrium constant, Keq, for the reaction ATP → ADP + Pi is 2.22 × 10⁵ M, calculate the standard free-energy change, ΔG°, for the synthesis of ATP from ADP and Pi at 25 °C.