To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 15922

#SCPH05 I Biotechnology

Exposure to carcinogens is a contributing factor for _________

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#Question id: 14116

#SCPH05 I Biotechnology

Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm? 

P. A larger E-value indicates higher sequence similarity 

Q. E-value < 10^-10 indicates sequence homology 

R. A higher BLAST score indicates higher sequence similarity 

S. E-value > 10^10 indicates sequence homology 

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#Question id: 11075

#SCPH28 | Zoology

A 65-year-old man with emphysema due to 34 years of cigarette smoking is admitted to hospital due to dyspnea. With further tests the mean pulmonary arterial pressure is determined to be 45 mm Hg at rest. He is hypoxic (PO2 = 49 mm Hg), hypercapnic (85 mm Hg), and slightly acidotic. The cardiovascular and oxygen changes are due to which of the following?

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#Question id: 1125

#SCPH28 | Zoology

Examples of G protein–coupled receptors include

a. rhodopsin receptor.             b. β-adrenergic receptor.

c. insulin receptor.                   d. acetylcholine receptor.

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#Question id: 330

#SCPH06 I Botany

Two charges are placed at a certain distance. If the magnitude of each charge is doubled the force will become