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TLS Online TPP Program

#Question id: 13106

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 
 
Why is it that some people only have one band?

#SCPH28 | Zoology
  1. They are homozygotes for the allele that can be cut.
  2. They are homozygotes for the allele that cannot be cut.
  3. They are heterozygotes, and possess one allele that can be cut and one allele that cannot be cut.
  4. The more repeats you have, the longer your DNA fragment.
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TLS Online TPP Program

#Question id: 27822

#Research Methodology

Which of the following sampling techniques is commonly used in quantitative research to ensure a representative sample?
TLS Online TPP Program

#Question id: 13054

#SCPH06 I Botany

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program

#Question id: 17915

#SCPH05 I Biotechnology

A dilatant fluid is one in which the viscosity
TLS Online TPP Program

#Question id: 12938

#I Life Science/ Life Sciences Group – I-V

What is the main advantage of using Yeast Artificial Chromosome based vector over other vectors?
1. It has ability to clone very large fragments of DNA.
2. YAC vectors do not contain origin of replication sites 
3. It has insert  range up to 2000 kb.
4. It help in genome mapping and sequencing projects.
TLS Online TPP Program

#Question id: 18381

#SCPH06 I Botany

Which bacterial strain was improved by scientists to be able to degrade 2,4-dinitrotoluene and promote plant growth