DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

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#Question id: 13106

#SCPH28 | Zoology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 

 

Why is it that some people only have one band?

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#Question id: 1611

#SCPH28 | Zoology

Which of the following is NOT produced following activation of the NADPH oxidase microbicidal pathway?

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#Question id: 13038

#SCPH28 | Zoology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 19655

#SCPH05 I Biotechnology

Which of the following statements about SN₂ mechanisms is incorrect?

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#Question id: 23151

#SCPH06 I Botany

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?

1. Fluorescence will decrease

2.  Fluorescence will increase

3. Emission of light at higher wavelength

4. Emission of light at lower wavelength

Which of the following options are correct?