TLS Online TPP Program

#Question id: 15620


Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition Lac– strain, called lac3–, is linked to the Tn5 insertion. From a strain carrying the Tn5 insertion and lac3– mutation you isolate an F’ that caries a region of the chromosome that includes both Tn5 and the linked Lac region. Introduce this F’ into an F– strain carrying lac1– by selecting for Kanr. These merodiploids express ß-galactosidase normally. What does this result tell you about the relationship between the lac3– and lac1- mutations?  

#SCPH28 | Zoology
  1. lac1- and lac3- mutations lie in different genes but not showing complement
  2.  lac1- and lac3- mutations lie in same gene
  3. lac1- and lac3- mutations are complement each other
  4. a and b