Which of cytokine is responsible for Class Switch IgM into IgE

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#Question id: 37

#SCPH28 | Zoology

Which one of the following has the cellular components arranged in order of their increasing size?

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#Question id: 20096

#SCPH05 I Biotechnology

If w1 , w2, w3 and w3 are work done in isothermal, adiabatic, isobaric and isochoric reversible process, the correct order (for expansion) will be

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#Question id: 633

#SCPH05 I Biotechnology

An amino acid transporter protein is responsible for the transport of a specific amino acid across a membrane. The KI values of several competitive inhibitors of the amino acid transporter are shown above. Based on these data, which of the following is most likely the amino acid transported by this protein?

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#Question id: 3529

#SCPH12 I Genetics

Mendel crossed tall and round seeded pea plants with dwarf and wrinkle seeded pea. The F1 plants were all tall and round seed. What is the probability of tall plant with wrinkle seed in F2 generation?

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#Question id: 13093

#SCPH05 I Biotechnology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.