Following are few sterilization techniques and materials used in plant tissue culture. Identify the correct combinations.

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#Question id: 13083

#SCPH06 I Botany

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 

(1) both ends cleaved by PstI (Pst-Pst), 

(2) both ends cleaved by MseI (Mse-Mse), and 

(3) one end generated by each of the two enzymes (Pst-Mse). 

the most frequent fragments in decreasing order,

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#Question id: 13083

#SCPH28 | Zoology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 

(1) both ends cleaved by PstI (Pst-Pst), 

(2) both ends cleaved by MseI (Mse-Mse), and 

(3) one end generated by each of the two enzymes (Pst-Mse). 

the most frequent fragments in decreasing order,

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#Question id: 13084

#SCPH01 Biochemistry

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

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#Question id: 13084

#SCPH05 I Biotechnology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

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#Question id: 13084

#SCPH06 I Botany

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13084

#SCPH28 | Zoology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.