The assembly of various DNA sequences designed for easy identification of the construct and its efficient expression is termed as

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#Question id: 13054

#SCPH06 I Botany

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 7760

#SCPH28 | Zoology

In general, the hormones that regulate senescence can be divided into two basic categories based on their most commonly observed effects:

I) positive (promoting) senescence regulators and

II) negative (repressing) senescence regulators

a) Hormones involved-Ethylene, Abscisic acid (ABA)

b) Hormones involved-Brassinosteroids (BRs), Salicylic acid (SA), Jasmonic acid (JA)

c) Hormones involved-Cytokinin, Auxin, Gibberellin (GAs)

Select correct combinations from given hormones which are involved in regulators?

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#Question id: 1541

#SCPH28 | Zoology

Nonphagocytic cell of immune system,

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#Question id: 1545

#SCPH05 I Biotechnology

B cells negatively regulated by,

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#Question id: 16486

#SCPH01 Biochemistry

A technique which has been successfully maintaining  long term culture upto 4-5 months is