According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 5434

#SCPH28 | Zoology

Plant height of wild Solanum nigrum is controlled by polygene. A pure line plant with 60 cm height was cross to pure line plant having 20 cm height .All F1 plant having uniform height 40 cm. F1 plant was allowed to self-fertilization to produced 4/64 proportional of F2 plant having height 20 cm . What is number of polygene governing plant height?

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#Question id: 19303

#SCPH01 Biochemistry

A typical plant gene has the following regions 

(i) Promoter

(ii) enhancer/silencer

(iii) Cap site 

(iv) Translated region  

(v) Initiation codon

(vi) Exons 

(vii) intron

(viii) Stop codon

(ix) Poly (A) tail

Which of the following option is incorrect?

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#Question id: 11476

#SCPH28 | Zoology

What structures would neurobiologists look for if they are interested in determining if an animal can see in color?

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#Question id: 2666

#SCPH06 I Botany

A cell does not want to waste energy synthesizing the amino acid arginine if arginine is present in its diet. Therefore, when arginine is present, it activates a repressor protein to prevent transcription of genes that code for enzyme to make arginine. This is an example of what type of regulation?

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#Question id: 5035

#SCPH01 Biochemistry

 A fertilized egg is called a: