Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

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#Question id: 18074

#SCPH06 I Botany

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use

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#Question id: 445

#SCPH06 I Botany

In order for a reaction to proceed spontaneously from left to right as written

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#Question id: 15654

#SCPH06 I Botany

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. Next you isolate a mutation, designated MelB–, which gives uninducible melibiase activity. Mapping experiments show that MelB– is linked to Mel1. Using an F' factor that carries the chromosomal region surrounding Mel1, you perform the following genetic tests:

                      

Describe the basic genetic properties of the MelB– mutation?

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#Question id: 13041

#SCPH05 I Biotechnology

Which of the following is incorrect about Data Collection? 

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#Question id: 1541

#I Life Science/ Life Sciences Group – I-V

Nonphagocytic cell of immune system,