Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 5707

#SCPH06 I Botany

Which of the following statement is correct for human is heterozygous for inversion?

#Question id: 2654

#SCPH06 I Botany

Regulation of the lac operon can be envisioned as regulation of enzyme synthesis by its

#Question id: 14269

#SCPH05 I Biotechnology

An activated-sludge waste treatment system is required to reduce the amount of BOD₅ from 1000 mg/l to 20 mg/l at the exit. The sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters are µ_m = 0.2 h-1 , K_s = 80 mg/l, k_d = 0.01 h-1 , and Y ^M _X/S = 0.5 g MLVSS/g BOD₅. The flow of waste water is 10000 l/h and the size of the treatme nt basin is 50,000 l. What value of the recycle ratio must be used?

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#Question id: 3653

#SCPH05 I Biotechnology

Cyclobutane pyrimidine dimers are formed by -------------from adjacent pyrimidines on one strand by cyclization of the double-bonded C5 and C6carbon atoms of each base to give a cyclobutane ring.

#Question id: 1036

#SCPH28 | Zoology

A urine sample with more than 100,000 organisms is considered indicative of infection. A urine sample containing 5,000 bacteria, with a generation time of 30 minutes, sits for 3 hours before finally being assayed. How many bacteria will then be present within the sample?