Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 19917

#SCPH01 Biochemistry

Antigen-Antibody interactions are__

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#Question id: 27706

#Research Methodology

……….. is “systematically conceptual structure of inter related elements in some schematic form”

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#Question id: 5550

#SCPH06 I Botany

The vertebrate nervous system is derived from

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#Question id: 1659

#I Life Science/ Life Sciences Group – I-V

Which of the following Fc receptors has the highest affinity?

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#Question id: 11962

#I Life Science/ Life Sciences Group – I-V

 In the digestive system, peristalsis is ________.