Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 19693

#SCPH06 I Botany

The U wave is an inconstant finding that:

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#Question id: 2722

#SCPH05 I Biotechnology

Which type of supercoiling takes the form of extended right-handed coils?

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#Question id: 5445

#SCPH28 | Zoology

A plant height in Maize is control by 3 pair of polygene .Two varieties of maize averaging 120 and 60 inches in height, respectively, are crossed. The F1 progeny is quite uniform averaging 90 inches in height. What is percent of F2 progeny that is resemble with either 120 cm or 60 cm height?

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#Question id: 15227

#SCPH06 I Botany

Electrophoresis of RNA is usually carried out in

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#Question id: 2286

#SCPH05 I Biotechnology

Bacterial flagella propel the cell by using