Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 10562

#SCPH05 I Biotechnology

The direction and rate of water flow across a membrane are determined by

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#Question id: 752

#SCPH28 | Zoology

The A260/A280 ratio of a double-stranded DNA sample can be used to assess its purity. The value for the pure DNA is ----------------

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#Question id: 14352

#SCPH05 I Biotechnology

The viscosity of a fluid changes with either stirrer speed or mixing time. But when mixing ceases, the viscosity returns to its original state. This fluid is best described as 

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#Question id: 27740

#Research Methodology

The process of quantifying data is referred to as ____________

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#Question id: 14119

#I Life Science/ Life Sciences Group – I-V

Which of the following is incorrect regarding sequence homology?