Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 5068

#I Life Science/ Life Sciences Group – I-V

In TGF-b signaling, the transcription factors downstream from the TGF-β receptors. These transcription factors in Drosophila and the related vertebrate proteins are called:

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#Question id: 15608

#SCPH06 I Botany

Melanins are light-absorbing biopolymers useful as topical sunscreens, sunlight-protective coatings for plastics, etc. Which gene is used for producing melanin. 

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#Question id: 358

#SCPH05 I Biotechnology

Consider the following reaction at equilibrium: CO2 + H2O ⇔ H2CO3. What would be the effect of adding additional H2O?

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#Question id: 3852

#SCPH28 | Zoology

Which of the following is NOT one of the three stages of transcription?

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#Question id: 5227

#SCPH06 I Botany

One of the characteristics of retrotransposons is that