TLS Online TPP Program

#Question id: 13061


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Arrange in the order of their increasing capacity:

#SCPH01 Biochemistry
  1. Plasmid, BAC, Replacement vectors, Insertion vectors.
  2. Replacement, BAC, Insertion, Plasmid
  3. BAC, Insertion, Replacement, Plasmid
  4. Plasmid, Insertion, Replacement, BAC
More Questions
TLS Online TPP Program

#Question id: 810

#SCPH05 I Biotechnology

Which of the following statements concerning the process of spontaneous folding of proteins is false?

TLS Online TPP Program

#Question id: 810

#SCPH28 | Zoology

Which of the following statements concerning the process of spontaneous folding of proteins is false?

TLS Online TPP Program

#Question id: 811

#SCPH01 Biochemistry

Protein S will fold into its native conformation only when protein Q is also present in the solution.  However, protein Q can fold into its native conformation without protein S.  Protein Q, therefore, may function as a ____________ for protein S.

TLS Online TPP Program

#Question id: 811

#I Life Science/ Life Sciences Group – I-V

Protein S will fold into its native conformation only when protein Q is also present in the solution.  However, protein Q can fold into its native conformation without protein S.  Protein Q, therefore, may function as a ____________ for protein S.

TLS Online TPP Program

#Question id: 811

#SCPH05 I Biotechnology

Protein S will fold into its native conformation only when protein Q is also present in the solution.  However, protein Q can fold into its native conformation without protein S.  Protein Q, therefore, may function as a ____________ for protein S.

TLS Online TPP Program

#Question id: 811

#SCPH28 | Zoology

Protein S will fold into its native conformation only when protein Q is also present in the solution.  However, protein Q can fold into its native conformation without protein S.  Protein Q, therefore, may function as a ____________ for protein S.