Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

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#Question id: 17084

#SCPH28 | Zoology

What color does phenol red turn in acid?

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#Question id: 17085

#SCPH01 Biochemistry

Spontaneous cell fusion occurs at a very low rate. Enhanced fusion can be done by 

1. Sendai virus 

2. Polyethylene glycol

3. Ficol

4. Phenol red

Which of the above mentioned can be used for fusion experiments?

TLS Online TPP Program

#Question id: 17085

#SCPH28 | Zoology

Spontaneous cell fusion occurs at a very low rate. Enhanced fusion can be done by 

1. Sendai virus 

2. Polyethylene glycol

3. Ficol

4. Phenol red

Which of the above mentioned can be used for fusion experiments?

TLS Online TPP Program

#Question id: 17196

#SCPH28 | Zoology

What happens when the stressor remains during the GAS?

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#Question id: 17197

#SCPH28 | Zoology

If a person does not find ways to manage stress levels at this stage, they are at risk of developing stress-related health conditions. This stage is known as:

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#Question id: 17198

#SCPH28 | Zoology

What is the role of endorphins?