Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

#Question id: 11601

#SCPH06 I Botany

Gibberellin is an important plant hormone that regulates several aspects of plant growth and development. Some statements are given below in relation to  signalling pathways:

a) The binding of gibberellin (GA) to its receptor leads to the degradation of the DELLA repressor by the 26S proteasome

b) Gibberellin binds to the GID1 receptor and induces a conformational change in the N-terminal domain of the receptor, allowing the receptor to interact with the TVHYNP and DELLA domains of the DELLA repressor

c) The formation of the GID1-repressor complex promotes the interaction between the repressor and the E3 ubiquitin ligase SCF^TIR1 complex, leading to the ubiquitination and degradation

d) The gibberellin receptor GIBBERELLIN INSENSITIVE DWARF 1 (GID1) also itself function as an F-box protein

Which one of the following is the incorrect statements about GA  signalling pathway;

#Question id: 11603

#I Life Science/ Life Sciences Group – I-V

Several important role promotes GA in the plant except____

#Question id: 11603

#SCPH06 I Botany

Several important role promotes GA in the plant except____

#Question id: 11604

#I Life Science/ Life Sciences Group – I-V

What will be the effect of exogenous gibberellin in plant?

a) extreme stem elongation in dwarf plants

b) Increases  in stem thickness

c) decrease in leaf size

d) decreases in stem thickness

e) Increases in leaf size

f) pale green color of the leaves

#Question id: 11604

#SCPH06 I Botany

What will be the effect of exogenous gibberellin in plant?

a) extreme stem elongation in dwarf plants

b) Increases  in stem thickness

c) decrease in leaf size

d) decreases in stem thickness

e) Increases in leaf size

f) pale green color of the leaves

#Question id: 11605

#I Life Science/ Life Sciences Group – I-V

Major uses of gibberellins applied as a spray or dip, are to manage several processes except,