TLS Online TPP Program

#Question id: 13083


The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

#SCPH01 Biochemistry
  1. Pst-Pst > Mse-Mse > Pst-Mse
  2. Mse-Mse > Pst-Mse > Pst-Pst
  3. Pst-Mse > Pst-Pst> Mse-Mse
  4. Mse-Mse > Pst-Pst > Pst-Mse
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TLS Online TPP Program

#Question id: 10788

#I Life Science/ Life Sciences Group – I-V

Phenyl ammonia lyase (PAL) involved in biosynthesis of phenolic compound in plants. Following are some statements regarding the action of PAL;

a) PAL catalyze conversion of phenyl alanine to p-coumaric acid

b) PAL catalyzes the conversion of phenyl alanine into trans-Cinnamic acid by elimination of an ammonia molecule

c) The activity of PAL is increased by environmental factors, such as low nutrient levels, light, and fungal infection

d) PAL is situated at a branch point between primary and secondary metabolism, so the reaction that it catalyzes is an important regulatory step in the formation of many phenolic compounds

Which of the following combinations of above statement is true?

TLS Online TPP Program

#Question id: 10788

#SCPH05 I Biotechnology

Phenyl ammonia lyase (PAL) involved in biosynthesis of phenolic compound in plants. Following are some statements regarding the action of PAL;

a) PAL catalyze conversion of phenyl alanine to p-coumaric acid

b) PAL catalyzes the conversion of phenyl alanine into trans-Cinnamic acid by elimination of an ammonia molecule

c) The activity of PAL is increased by environmental factors, such as low nutrient levels, light, and fungal infection

d) PAL is situated at a branch point between primary and secondary metabolism, so the reaction that it catalyzes is an important regulatory step in the formation of many phenolic compounds

Which of the following combinations of above statement is true?

TLS Online TPP Program

#Question id: 10789

#I Life Science/ Life Sciences Group – I-V

Trans-cinnamic acid, p-coumaric acid, and their derivatives are simple phenolic compounds known as

TLS Online TPP Program

#Question id: 10789

#SCPH05 I Biotechnology

Trans-cinnamic acid, p-coumaric acid, and their derivatives are simple phenolic compounds known as

TLS Online TPP Program

#Question id: 10790

#I Life Science/ Life Sciences Group – I-V

chalcone synthase (CHS) involved in biosynthesis of phenolic compound in plants. Following are some statements regarding the action of CHS;

 a) Chalcone synthase catalyzes the conversion of p-Coumaroyl-CoA into Chalcones

 b) Substrate for Chalcone synthase is chalcone

 c) CHS activity increased by environmental factors, such as light, and fungal infection

 d) Chalcone synthase is enzyme in flavonoid biosynthesis catalyzes stepwise condensation into p-Coumaric acid by using Malonyl-CoA molecules

 Which of the following combinations of above statement is true?

TLS Online TPP Program

#Question id: 10790

#SCPH05 I Biotechnology

chalcone synthase (CHS) involved in biosynthesis of phenolic compound in plants. Following are some statements regarding the action of CHS;

 a) Chalcone synthase catalyzes the conversion of p-Coumaroyl-CoA into Chalcones

 b) Substrate for Chalcone synthase is chalcone

 c) CHS activity increased by environmental factors, such as light, and fungal infection

 d) Chalcone synthase is enzyme in flavonoid biosynthesis catalyzes stepwise condensation into p-Coumaric acid by using Malonyl-CoA molecules

 Which of the following combinations of above statement is true?