To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

#Question id: 10547

#SCPH05 I Biotechnology

Which of the following responses are incorrect for closing of stomata?

#Question id: 10548

#SCPH05 I Biotechnology

flg22-elicited response is dependent on the presence of the LRR receptor-like kinase, what will be the causes of mutant form of LRR receptor-like kinase FLS2?

#Question id: 10549

#I Life Science/ Life Sciences Group – I-V

Following are the list of some of the molecular “danger signals” (column I) and its characteristics (column II)                                                                                         

 Column I                                                                                   Column II

 a) MAMPs                               i) produce from the pathogen molecules support their ability to successfully colonize their host and gain nutritional benefits

b) DAMPs                               ii) A plant response to insect herbivores involve both a wound response and the recognition of certain compounds abundant in the insect’s saliva or regurgitant

c) Effectors                             iii) are conserved among a specific class of microorganisms such as chitin for fungi, flagella for bacteria

d) Elicitor                                iv) molecules secrete from the damaging plant by herbivory such as Prosystemin

Which of the following is the correct match between the signals and their respective mechanism?

#Question id: 10549

#SCPH05 I Biotechnology

Following are the list of some of the molecular “danger signals” (column I) and its characteristics (column II)                                                                                         

 Column I                                                                                   Column II

 a) MAMPs                               i) produce from the pathogen molecules support their ability to successfully colonize their host and gain nutritional benefits

b) DAMPs                               ii) A plant response to insect herbivores involve both a wound response and the recognition of certain compounds abundant in the insect’s saliva or regurgitant

c) Effectors                             iii) are conserved among a specific class of microorganisms such as chitin for fungi, flagella for bacteria

d) Elicitor                                iv) molecules secrete from the damaging plant by herbivory such as Prosystemin

Which of the following is the correct match between the signals and their respective mechanism?

#Question id: 10550

#SCPH05 I Biotechnology

Plants, in turn, evolved a second line of defense that recognize these intracellular effectors and trigger defense responses to render them harmless. As a result, plants possess a second type of immunity called,

#Question id: 10551

#I Life Science/ Life Sciences Group – I-V

plants possess a second type of immunity mediated by a set of highly specific intracellular receptors known as nucleotide binding site–leucine rich repeat (NBS–LRR) receptors. How NBS–LRRs become activated,