To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;

#Question id: 4811

#SCPH28 | Zoology

Three incompletely link gene as a B-A-C order. A cross allowed between AAbbcc X aaBBCC produce f1 progeny allowed test cross. Which of the following offspring is a result of double cross over?

#Question id: 4812

#SCPH06 I Botany

Two character X and Y ,both follow law of dominance due to 3:1 F2 phenotypic ratio monohybrid cross separately If carry out dihybrid selfing cross for heterozygous genotype for both character does not follow law of independence assortment in normal environmental condition due to?

#Question id: 4812

#SCPH12 I Genetics

Two character X and Y ,both follow law of dominance due to 3:1 F2 phenotypic ratio monohybrid cross separately If carry out dihybrid selfing cross for heterozygous genotype for both character does not follow law of independence assortment in normal environmental condition due to?

#Question id: 4812

#SCPH28 | Zoology

Two character X and Y ,both follow law of dominance due to 3:1 F2 phenotypic ratio monohybrid cross separately If carry out dihybrid selfing cross for heterozygous genotype for both character does not follow law of independence assortment in normal environmental condition due to?

#Question id: 4813

#SCPH06 I Botany

You perform the three different two-factor crosses (Cross 1: XY and xy, Cross 2: YZ and yz, and Cross 3: XZ and xz). Assume all crosses are between diploid flies homozygous for the alleles of these genes. You observe 7% recombinants in the first cross, 20% recombinants in the second cross, and 13% recombinants in the third cross. if cross between XyZ and xYz leads to which of the followining recombinant with double cross over .

#Question id: 4813

#SCPH12 I Genetics

You perform the three different two-factor crosses (Cross 1: XY and xy, Cross 2: YZ and yz, and Cross 3: XZ and xz). Assume all crosses are between diploid flies homozygous for the alleles of these genes. You observe 7% recombinants in the first cross, 20% recombinants in the second cross, and 13% recombinants in the third cross. if cross between XyZ and xYz leads to which of the followining recombinant with double cross over .