To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 11273

#SCPH06 I Botany

Use the following diagram of a hypothetical food web to answer the question. The arrows represent the transfer of energy between the various trophic levels. Which letter represents an organism that could only be a primary consumer and secondary carnivores respectively?

#Question id: 11273

#SCPH28 | Zoology

Use the following diagram of a hypothetical food web to answer the question. The arrows represent the transfer of energy between the various trophic levels. Which letter represents an organism that could only be a primary consumer and secondary carnivores respectively?

#Question id: 11274

#SCPH06 I Botany

Assimilation efficiency (AE) is the proportion of ingested energy assimilated in to blood stream but Production efficiency (PE) is the proportion of assimilated energy that is incorporated in to biomass. Following some statement is correct?

A. Assimilation efficiencies of aquatic herbivore is greater than terrestrial herbivore

B. Production efficiencies of higher tropic level are greater than lower tropic level

C. production efficiencies of vertebrate is less than invertebrate

D. Assimilation efficiencies of Carnivore are often greater than herbivore

#Question id: 11274

#SCPH28 | Zoology

Assimilation efficiency (AE) is the proportion of ingested energy assimilated in to blood stream but Production efficiency (PE) is the proportion of assimilated energy that is incorporated in to biomass. Following some statement is correct?

A. Assimilation efficiencies of aquatic herbivore is greater than terrestrial herbivore

B. Production efficiencies of higher tropic level are greater than lower tropic level

C. production efficiencies of vertebrate is less than invertebrate

D. Assimilation efficiencies of Carnivore are often greater than herbivore

#Question id: 11275

#SCPH06 I Botany

Consumption efficiency is the proportion of production at one trophic level that is eaten by, or ingested, by the next trophic level which of the following are correct statement ?

A.Consumption efficiencies higher in  forests than grassland

B-In aquatic ecosystems consuption efficiencies of hervivore are greater than terrestrial hervivore

C- Consumption efficiencies for carnivores are typically higher than those of herbivores

D-Primary production consumption is increased where it is high in nutrients such as nitrogen and phosphorus

#Question id: 11275

#SCPH28 | Zoology

Consumption efficiency is the proportion of production at one trophic level that is eaten by, or ingested, by the next trophic level which of the following are correct statement ?

A.Consumption efficiencies higher in  forests than grassland

B-In aquatic ecosystems consuption efficiencies of hervivore are greater than terrestrial hervivore

C- Consumption efficiencies for carnivores are typically higher than those of herbivores

D-Primary production consumption is increased where it is high in nutrients such as nitrogen and phosphorus