To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

TLS Online TPP Program

#Question id: 14156

#SCPH01 Biochemistry

A short stretch of identical residues in FASTA is called 

TLS Online TPP Program

#Question id: 14156

#I Life Science/ Life Sciences Group – I-V

A short stretch of identical residues in FASTA is called 

TLS Online TPP Program

#Question id: 14156

#SCPH05 I Biotechnology

A short stretch of identical residues in FASTA is called 

TLS Online TPP Program

#Question id: 14156

#SCPH05 I Biotechnology

A short stretch of identical residues in FASTA is called 

TLS Online TPP Program

#Question id: 14157

#SCPH01 Biochemistry

A ktup is composed of ___residues for protein sequences and _____ residues for DNA sequences.

TLS Online TPP Program

#Question id: 14157

#I Life Science/ Life Sciences Group – I-V

A ktup is composed of ___residues for protein sequences and _____ residues for DNA sequences.