You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                     

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.

 

a) Pattern-1, DV only + Ligase→No colonies b/c you have digested with 2 different restriction enzymes that can’t ligate together 

b) Pattern-2, DYG only + Ligase→ No colonies because all you transformed is the digested, linear yeast DNA.

c) Pattern-3, Water + Ligase→ No plasmid with the ampicillin resistance gene (or any DNA) was transformed into the bacteria and so it won’t grow in the presence of ampicillin.

d)Pattern-3, DV + DYG + Ligase→Colonies. The plasmid and yeast gene can ligate together to form a functional plasmid that will express the ampicillin resistance gene.

e) Pattern-1 and 2 only, DV + DYG (No Ligase) →No colonies because, although you have both digested plasmid and a digested yeast gene with complementary sticky ends

Which of the following statements about these ligations and their pattern is correct?

#Question id: 9282

#SCPH06 I Botany

The rate of photosynthesis when red and far-red light are given together is greater than the sum of the rates when they are given apart, the effect provided essential

evidence in favor of the concept that photosynthesis is carried out by two photochemical systems working in tandem but with slightly different wavelength optima

this effect is known as

#Question id: 9283

#SCPH05 I Biotechnology

Organization of the protein complexes the photosystems I and II Are spatially separated in the thylakoid membrane, choose the correct arrangement of PSI and PSII on the membrane ;

a.) The PSI reaction centre and its associated antenna pigments and electron transfer proteins, as well as the coupling-factor enzyme that catalyzes the formation of ATP, are found almost exclusively in the stroma lamellae and at the edges of the grana lamellae

b.) The PSI reaction centre, along with its antenna chlorophylls and associated electron transport proteins, is located predominantly in the stromal lamellae

c.) The PSII reaction centre and its associated antenna pigments and electron transfer proteins, as well as the coupling-factor enzyme that catalyzes the formation of ATP, are found almost exclusively in the stroma lamellae and at the edges of the grana lamellae

d.) The PSII reaction centre, along with its antenna chlorophylls and associated electron transport proteins, is located predominantly in the grana lamellae

Which of the following given combination correct?

#Question id: 9283

#SCPH06 I Botany

Organization of the protein complexes the photosystems I and II Are spatially separated in the thylakoid membrane, choose the correct arrangement of PSI and PSII on the membrane ;

a.) The PSI reaction centre and its associated antenna pigments and electron transfer proteins, as well as the coupling-factor enzyme that catalyzes the formation of ATP, are found almost exclusively in the stroma lamellae and at the edges of the grana lamellae

b.) The PSI reaction centre, along with its antenna chlorophylls and associated electron transport proteins, is located predominantly in the stromal lamellae

c.) The PSII reaction centre and its associated antenna pigments and electron transfer proteins, as well as the coupling-factor enzyme that catalyzes the formation of ATP, are found almost exclusively in the stroma lamellae and at the edges of the grana lamellae

d.) The PSII reaction centre, along with its antenna chlorophylls and associated electron transport proteins, is located predominantly in the grana lamellae

Which of the following given combination correct?

#Question id: 9284

#SCPH05 I Biotechnology

Following are some facts regarding spatial separation of PSI and PSII. Which one would be INCORRECT?

#Question id: 9284

#SCPH06 I Botany

Following are some facts regarding spatial separation of PSI and PSII. Which one would be INCORRECT?

#Question id: 9285

#SCPH05 I Biotechnology