TLS Online TPP Program

#Question id: 13107


With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

#SCPH01 Biochemistry
  1. The more repeats you have, the longer your DNA fragment is, and longer DNA fragments migrate more slowly in gels.
  2. They are homozygotes for the allele that cannot be cut.
  3. They are homozygotes for the allele that can be cut.
  4. They are heterozygotes, and possess one allele that can be cut and one allele that cannot be cut.
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TLS Online TPP Program

#Question id: 15275

#SCPH28 | Zoology

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use

TLS Online TPP Program

#Question id: 15276

#SCPH01 Biochemistry

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 15276

#SCPH06 I Botany

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 15276

#SCPH28 | Zoology

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

TLS Online TPP Program

#Question id: 15277

#SCPH01 Biochemistry

Although PCR and replication show resemblances, but the basic difference is

TLS Online TPP Program

#Question id: 15277

#SCPH06 I Botany

Although PCR and replication show resemblances, but the basic difference is