Sieving effect that contributes to the separation of proteins according to their size, in techniques such as SDS–gel electrophoresis, the concentration of gel used

#Question id: 2606

#I Life Science/ Life Sciences Group – I-V

Activity of trp operon is taken as a measurement of ;

#Question id: 486

#SCPH05 I Biotechnology

The ultimate electron acceptor in the fermentation of glucose to ethanol is:

#Question id: 15635

#I Life Science/ Life Sciences Group – I-V

#Question id: 11603

#I Life Science/ Life Sciences Group – I-V

Several important role promotes GA in the plant except____

#Question id: 13076

#SCPH06 I Botany

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.