#Question id: 642
#SCPH05 I Biotechnology
The graphs P, Q and R show the variation of rate constant (k) with temperature. The reactions represented by P, Q and R, respectively, are
#Question id: 642
#SCPH05 I Biotechnology
The graphs P, Q and R show the variation of rate constant (k) with temperature. The reactions represented by P, Q and R, respectively, are
#Question id: 643
#SCPH05 I Biotechnology
The hydrolysis of lactose is catalysed by b-galactosidase as given below
Which of the following could be correct mechanism for this hydrolysis reaction?
A. Nucleophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
B. Electrophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
C. Nucleophilic attack by the carboxylate group of Lys/Arg/His or bivalent cation on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
#Question id: 644
#SCPH05 I Biotechnology
Enzyme X and Enzyme Y are both involved in monosaccharide metabolism. Enzyme X uses glucose as a substrate while Enzyme Y uses fructose as a substrate. At pH=7.0, Enzyme X has a Vmax of 10 μM/s while Enzyme Y has a Vmax of 20 μM/s. Both enzymes have a Km of 3.0 mM for their respective substrates. Which aspects of its reaction will be changed by Enzyme Y?
#Question id: 654
#SCPH01 Biochemistry
Which pair represents different conformations?
#Question id: 654
#I Life Science/ Life Sciences Group – I-V
Which pair represents different conformations?