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TLS Online TPP Program

#Question id: 15652

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation in terms of its basic genetic properties, 

#SCPH01 Biochemistry
  1.  MelA- as a recessive, loss of function mutation, whose wild-type allele codes for a cis-acting factor.
  2. MelA- as a recessive, loss of function mutation, whose wild-type allele codes for a trans-acting factor.
  3. MelA- as a dominant, gain of function mutation, whose wild-type allele codes for a trans-acting factor.
  4. MelA- as a dominant, gain of function mutation, whose wild-type allele codes for a cis-acting factor.
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TLS Online TPP Program

#Question id: 14265

#SCPH05 I Biotechnology

For the activated-sludge, the specific growth rate of cells is given by 


 

The following parameter values are known: F = 500 l/h, α = 0.4, ɣ = 0.1, Xe = 0, V = 1500 l, Ks = 10 mg/l, µm = 1 h-1 , kd = 0.05 h-1 , S0 = 1000 mg/l, Y M X/S = 0.5 g dw/g substrate. Calculate the substrate concentration (S) in the reactor at steady state. -------------------------
TLS Online TPP Program

#Question id: 14266

#SCPH05 I Biotechnology

For the activated-sludge, the specific growth rate of cells is given by 


 

The following parameter values are known: F = 500 l/h, α = 0.4, ɣ = 0.1, Xe = 0, V = 1500 l, Ks = 10 mg/l, µm = 1 h-1 , kd = 0.05 h^-1 , S0 = 1000 mg/l, Y M X/S = 0.5 g dw/g substrate.. Calculate the cell concentration in the reactor. 
TLS Online TPP Program

#Question id: 14267

#SCPH05 I Biotechnology

In a trickling biological filter, the BOD value of the feed stream is S0i = 500 mg/l with a feed flow of F = 103 l/h. The effluent BOD value is desired to be S0 = 10 mg/l. The following kinetic parameters for the biocatalysts are known: rm = 20 mg/S/l .h and Ks = 200 mg S/l. The biofilm thickness is L = 0.1 mm. The cross-sectional area of the filter is A = 2 m2 , and the biofilm surface area per unit volume of the bed is a = 500 cm2 /cm3 . Assume that dissolved oxygen is the rate-limiting substrate and the diffusion coefficient of oxygen is DO2 = 2 × 10-5 cm2 /s. Determine the required height of the bed. You can assume first-order bioreaction kinetics?

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TLS Online TPP Program

#Question id: 14268

#SCPH05 I Biotechnology

An activated-sludge waste treatment system is required to reduce the amount of BOD5 from 1000 mg/l to 20 mg/l at the exit. The sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters are µm = 0.2 h-1 , Ks = 80 mg/l, kd = 0.01 h-1 , and Y M X/S = 0.5 g MLVSS/g BOD5. The flow of waste water is 10000 l/h and the size of the treatment basin is 50,000 l. What is the value of the solids residence time?
TLS Online TPP Program

#Question id: 14269

#SCPH05 I Biotechnology

An activated-sludge waste treatment system is required to reduce the amount of BOD5 from 1000 mg/l to 20 mg/l at the exit. The sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters are µm = 0.2 h-1 , Ks = 80 mg/l, kd = 0.01 h-1 , and Y M X/S = 0.5 g MLVSS/g BOD5. The flow of waste water is 10000 l/h and the size of the treatme nt basin is 50,000 l. What value of the recycle ratio must be used?

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TLS Online TPP Program

#Question id: 14270

#SCPH05 I Biotechnology

Consider a well-mixed waste treatment system for a small-scale system. The system is operated with a reactor of 1000 l and flow rate of 100 l/h. The separator concentrates biomass by a factor of 2. The recycle ratio is 0.7. The kinetic parameters are µm = 0.5 h-1 , Ks = 0.2 g/l, Y M X/S = 0.5 g/g, and kd = 0.05 h-1 . What is the exit substrate concentration?