5 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.

Describe the cis-acting elements in the GLN1 promoter that are evident from these experiments, giving both their position and as much of their function as you can deduce.

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#Question id: 14279

#SCPH05 I Biotechnology

Growth yield of mDicrobes is defined as

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#Question id: 14280

#SCPH05 I Biotechnology

In microbial cultivation, the expression for product synthesis

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#Question id: 14280

#SCPH05 I Biotechnology

In microbial cultivation, the expression for product synthesis

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#Question id: 14281

#SCPH05 I Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 

if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

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#Question id: 14281

#SCPH05 I Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 

if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

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#Question id: 14282

#SCPH05 I Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the