TLS Online TPP Program

#Question id: 16136


You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
Is there a second STS at which you would like to sequence PCR products obtained using BACs as templates? If so, which BACs would you test in this way, and what sequencing results might you predict for each of the BACs tested?

#SCPH01 Biochemistry
  1. STS1 is present more than once; BACs A, B,  would have the same sequence C, E, F would have the same sequence.
  2. STS5 is present more than once; BACs B, C, D, E, and F would have the same sequence.
  3. STS1 is present more than once; BACs A, B, C would have the same sequence E, F would have the same sequence.
  4. STS2 is present more than once; BACs B, C, D would have the different sequence.