The PCR consists of three defined sets of times and temperatures, termed steps

a) Denaturation at 94°C, for 0.5 min

b) Primer annealing at 55°C for 1.5 min

c) Extension at 72°C for 1 min

d) only denaturation and extension

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#Question id: 18707

#SCPH01 Biochemistry

primer dimers are formed when 

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#Question id: 18708

#SCPH01 Biochemistry

PCR can be analysed amplification, Anneleaning, and extension and analysis is called

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#Question id: 18709

#SCPH01 Biochemistry

qPCR can be done by 

i) cyanin dye or SYBR green – to measure amplification

ii) Taqman assay – measure extension

iii) molecular beacons - denaturation

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#Question id: 18710

#SCPH01 Biochemistry

qPCR  graph is drawn between ∆Rn and PCR cycle In this graph CT represents

 

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#Question id: 18711

#SCPH01 Biochemistry

SYBR green qPCR based on 

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#Question id: 18712

#SCPH01 Biochemistry

which of the following are two major functions of RE